single guide rna sgrna expression plasmids Search Results


zo-1 small guide rna (sgrna) plasmid  (Genechem)
90
Genechem zo-1 small guide rna (sgrna) plasmid
Zo 1 Small Guide Rna (Sgrna) Plasmid, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zo-1 small guide rna (sgrna) plasmid/product/Genechem
Average 90 stars, based on 1 article reviews
zo-1 small guide rna (sgrna) plasmid - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
plasmids expressing single guide rna (sgrna  (Azenta)
90
Azenta plasmids expressing single guide rna (sgrna
TSP scp promotes gene activation activity of CRISPR-dCas system. ( A ) Activation of mNG expression by <t>mCherry-dCas12f-VPR/sgRNA</t> targeting mNG promoter in the HEK293T reporter cells after TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfection. In the non-targeted (NT) sgRNA control, cells transfected with a non-targeted sgRNA. Fluorescence imaging and flow cytometry analysis were performed 48 h post transfection. Red, mCherry-dCas12f-VPR. Green, mNG. VPR (VP64-p65-Rta), a transcriptional activator. Scale bar, 250 μm. ( B ) Schematic analysis of endogenous gene activation by CRISPR-dCas12a delivered by TSP scp and other transfection reagents. ( C-D ) qPCR analysis of targeted genes including HBG, IL1RN and TTN, in TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfected cells. <t>RNA</t> extraction was performed 48 h post transfection. n = 3 per group. Data represents as mean ± SD, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. P -values are from ANNOVA analysis and Sidak’s multiple comparisons test
Plasmids Expressing Single Guide Rna (Sgrna, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmids expressing single guide rna (sgrna/product/Azenta
Average 90 stars, based on 1 article reviews
plasmids expressing single guide rna (sgrna - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


TSP scp promotes gene activation activity of CRISPR-dCas system. ( A ) Activation of mNG expression by mCherry-dCas12f-VPR/sgRNA targeting mNG promoter in the HEK293T reporter cells after TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfection. In the non-targeted (NT) sgRNA control, cells transfected with a non-targeted sgRNA. Fluorescence imaging and flow cytometry analysis were performed 48 h post transfection. Red, mCherry-dCas12f-VPR. Green, mNG. VPR (VP64-p65-Rta), a transcriptional activator. Scale bar, 250 μm. ( B ) Schematic analysis of endogenous gene activation by CRISPR-dCas12a delivered by TSP scp and other transfection reagents. ( C-D ) qPCR analysis of targeted genes including HBG, IL1RN and TTN, in TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfected cells. RNA extraction was performed 48 h post transfection. n = 3 per group. Data represents as mean ± SD, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. P -values are from ANNOVA analysis and Sidak’s multiple comparisons test

Journal: Journal of Nanobiotechnology

Article Title: Short cell-penetration peptide conjugated bioreducible polymer enhances gene editing of CRISPR system

doi: 10.1186/s12951-024-02554-w

Figure Lengend Snippet: TSP scp promotes gene activation activity of CRISPR-dCas system. ( A ) Activation of mNG expression by mCherry-dCas12f-VPR/sgRNA targeting mNG promoter in the HEK293T reporter cells after TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfection. In the non-targeted (NT) sgRNA control, cells transfected with a non-targeted sgRNA. Fluorescence imaging and flow cytometry analysis were performed 48 h post transfection. Red, mCherry-dCas12f-VPR. Green, mNG. VPR (VP64-p65-Rta), a transcriptional activator. Scale bar, 250 μm. ( B ) Schematic analysis of endogenous gene activation by CRISPR-dCas12a delivered by TSP scp and other transfection reagents. ( C-D ) qPCR analysis of targeted genes including HBG, IL1RN and TTN, in TSP scp /pDNA, TSP/pDNA, PEI/pDNA and Lipo/pDNA transfected cells. RNA extraction was performed 48 h post transfection. n = 3 per group. Data represents as mean ± SD, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001. P -values are from ANNOVA analysis and Sidak’s multiple comparisons test

Article Snippet: Plasmids expressing single guide RNA (sgRNA) were constructed by ligating the corresponding annealed oligos to the basic plasmid under the human U6 promoter (GENEWIZ, China).

Techniques: Activation Assay, Activity Assay, CRISPR, Expressing, Transfection, Fluorescence, Imaging, Flow Cytometry, RNA Extraction